Isolation and characterization of partially purified leptospiral antigens
نویسندگان
چکیده
منابع مشابه
Preparation and in-vitro characterization of alginate microspheres incorporating leptospiral antigens as a delivery system and adjuvant
Leptospirosis is one of the most prevalent zoonotic diseases worldwide. Currently, multivalent whole-cell leptospiral vaccines can induce protection against leptospirosis. Therefore, preparation and formulation of new generations of vaccines that can stimulate long-term immunity for leptospirosis control are essential. The aim of this study was to prepare and characterize alginate microspheres ...
متن کاملsynthesis and characterization of some macrocyclic schiff bases
ماکروسیکلهای شیف باز از اهمیت زیادی در شیمی آلی و دارویی برخوردار می باشند. این ماکروسیکلها با دارابودن گروه های مناسب در مکانهای مناسب می توانند فلزاتی مثل مس، نیکل و ... را در حفره های خود به دام انداخته، کمپلکسهای پایدار تولید نمایند. در این پایان نامه ابتدا یک دی آلدئید آروماتیک از گلیسیرین تهیه می شود و در مرحله بعدی واکنش با دی آمینهای آروماتیک و یا آلیفاتیک در رقتهای بسیار زیاد منجر به ت...
15 صفحه اولStructural characterization of H-2 antigens purified from mouse liver.
Papain solubilized H-2a histocompatibility antigens (H-2Kk plus H-2Dd) have been purified by a large-scale procedure that can routinely provide 2-3 mg of heavy chain from 1 kg of mouse liver. The heavy chains were homogeneous by sodium dodecyl sulfate electrophoresis. Disc gel electrophoresis resolved two protein bands that were identified as H-2Dd and H-2Kd by immune complex formation and auto...
متن کاملIsolation, Characterization and Optimization of Process Variables for Partially Purified Immobilized Α-amylase Isolated from Indian Chicken Feather
Immobilization of α-Amylase produced by a Pseudomonas sp isolated from Indian Chicken feather was studied. Partially purified enzyme with 486.77 IU and specific activity of 811.28 units mg (protein) was used for immobilization study. An easily available and inexpensive 3% Sodium alginate matrix with easy immobilization gel entrapment procedure was used for trapping the enzyme showed 555.5 IU ac...
متن کاملRapid and simplified protocol for isolation and characterization of leptospiral chromosomal DNA for taxonomy and diagnosis.
We have developed a rapid method for the isolation of leptospiral chromosomal DNA which yields DNA of a purity suitable for restriction endonuclease analysis. A small volume (15 to 20 ml) of an exponentially growing culture of leptospires yielded 2 to 4 micrograms of chromosomal DNA. In a 1-day protocol, the DNA was isolated, restricted with endonucleases, and fractionated on an agarose gel. Ch...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Revista do Instituto de Medicina Tropical de São Paulo
سال: 1991
ISSN: 0036-4665
DOI: 10.1590/s0036-46651991000500009